Real-time determination of aggregated alpha-synuclein induced membrane disruption at neuroblastoma cells using scanning ion conductance microscopy
- Journal: Faraday Discussions
- Published Year: 2018
- Authors: Stephanie Wong Su, Andy Chieng, Jacob Parres-Gold, Megan Chang, Yixian Wang
- Application: neurodegenerative disease research, advanced bioimaging
Summary
In this study, Wong Su et al. used scanning ion conductance microscopy (SICM) to study real-time effects of aggregated alpha-synuclein (a-Syn) on neuroblastoma cell membranes. SICM imaging, performed with a Park NX12 AFM, revealed pore-like defects and morphological disruptions caused by both oligomeric and fibrillar a-Syn. Complementary AFM (non-contact, tapping mode) confirmed aggregate size and structure. SICM enabled non-invasive, nanometer-resolution visualization of dynamic membrane damage. This method provides a direct, label-free approach to studying a-Syn cytotoxicity, relevant to Parkinson’s disease pathology, and could extend to research in other neurodegenerative disorders.
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